Stochastic to Deterministic: A straightforward approach to create serially perfusable multiscale capillary beds.

TitleStochastic to Deterministic: A straightforward approach to create serially perfusable multiscale capillary beds.
Publication TypeJournal Article
Year of Publication2024
AuthorsDonzanti MJ, Mhatre O, Chernokal B, Renteria DC, Gleghorn JP
JournalbioRxiv
Date Published2024 May 06
ISSN2692-8205
Abstract

Generation of in vitro tissue models with serially perfused hierarchical vasculature would allow greater control of fluid perfusion throughout the network and enable direct mechanistic investigation of vasculogenesis, angiogenesis, and vascular remodeling. In this work, we have developed a method to produce a closed, serially perfused, multiscale vessel network embedded within an acellular hydrogel. We confirmed that the acellular and cellular gel-gel interface was functionally annealed without preventing or biasing cell migration and endothelial self-assembly. Multiscale connectivity of the vessel network was validated via high-resolution microscopy techniques to confirm anastomosis between self-assembled and patterned vessels. Lastly, using fluorescently labeled microspheres, the multiscale network was serially perfused to confirm patency and barrier function. Directional flow from inlet to outlet man-dated flow through the capillary bed. This method for producing closed, multiscale vascular networks was developed with the intention of straightforward fabrication and engineering techniques so as to be a low barrier to entry for researchers who wish to investigate mechanistic questions in vascular biology. This ease of use offers a facile extension of these methods for incorporation into organoid culture, organ-on-a-chip (OOC) models, and bioprinted tissues.

DOI10.1101/2024.05.03.592474
Alternate JournalbioRxiv
PubMed ID38766003
PubMed Central IDPMC11100595

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