Noncovalent interactions with SUMO and ubiquitin orchestrate distinct functions of the SLX4 complex in genome maintenance.

TitleNoncovalent interactions with SUMO and ubiquitin orchestrate distinct functions of the SLX4 complex in genome maintenance.
Publication TypeJournal Article
Year of Publication2015
AuthorsOuyang J, Garner E, Hallet A, Nguyen HDang, Rickman KA, Gill G, Smogorzewska A, Zou L
JournalMol Cell
Volume57
Issue1
Pagination108-22
Date Published2015 Jan 08
ISSN1097-4164
KeywordsAmino Acid Sequence, Animals, Cell Line, Tumor, DNA Damage, Escherichia coli, Gene Expression Regulation, Genome, HEK293 Cells, Humans, Mice, Molecular Sequence Data, Mutation, Protein Binding, Protein Interaction Domains and Motifs, Recombinant Fusion Proteins, Recombinases, Sequence Alignment, Signal Transduction, Small Ubiquitin-Related Modifier Proteins, Telomere, Ubiquitin, Ubiquitin-Conjugating Enzymes, Ubiquitins, Ultraviolet Rays
Abstract

SLX4, a coordinator of multiple DNA structure-specific endonucleases, is important for several DNA repair pathways. Noncovalent interactions of SLX4 with ubiquitin are required for localizing SLX4 to DNA interstrand crosslinks (ICLs), yet how SLX4 is targeted to other functional contexts remains unclear. Here, we show that SLX4 binds SUMO-2/3 chains via SUMO-interacting motifs (SIMs). The SIMs of SLX4 are dispensable for ICL repair but important for processing CPT-induced replication intermediates, suppressing fragile site instability, and localizing SLX4 to ALT telomeres. The localization of SLX4 to laser-induced DNA damage also requires the SIMs, as well as DNA end resection, UBC9, and MDC1. Furthermore, the SUMO binding of SLX4 enhances its interaction with specific DNA-damage sensors or telomere-binding proteins, including RPA, MRE11-RAD50-NBS1, and TRF2. Thus, the interactions of SLX4 with SUMO and ubiquitin increase its affinity for factors recognizing different DNA lesions or telomeres, helping to direct the SLX4 complex in distinct functional contexts.

DOI10.1016/j.molcel.2014.11.015
Alternate JournalMol. Cell
PubMed ID25533185
PubMed Central IDPMC4289429
Grant ListGM076388 / GM / NIGMS NIH HHS / United States
R01 HL120922 / HL / NHLBI NIH HHS / United States
T32GM007739 / GM / NIGMS NIH HHS / United States
R01 GM076388 / GM / NIGMS NIH HHS / United States
R01HL120922 / HL / NHLBI NIH HHS / United States
T32 GM007739 / GM / NIGMS NIH HHS / United States

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