Title | Traumatic injury induces interleukin-6 production by human astrocytes. |
Publication Type | Journal Article |
Year of Publication | 1994 |
Authors | Hariri RJ, Chang VA, Barie PS, Wang RS, Sharif SF, Ghajar JB |
Journal | Brain Res |
Volume | 636 |
Issue | 1 |
Pagination | 139-42 |
Date Published | 1994 Feb 04 |
ISSN | 0006-8993 |
Keywords | Astrocytes, Atmospheric Pressure, Brain Injuries, Cell Division, Cell Line, Humans, Interleukin-6 |
Abstract | The brain is being evaluated as a de novo source of cytokines. Because recent evidence indicates that interleukin-6 (IL-6) may influence blood-brain barrier function and vascular permeability, we have sought to determine whether mechanical injury can directly induce in situ cerebral IL-6 production. Adult human astrocyte cultures were subjected to mechanical injury by the in vitro method of fluid percussion barotrauma, developed in our laboratory. Serial supernatant samples were collected for 8 h and evaluated for IL-6 activity using a proliferation assay employing the dependent B cell hybridoma cell line, B9. At optimum injury, the IL-6 level became significantly (P < 0.0001, analysis of variance) elevated from baseline 2 h after trauma and continued to increase over the observation period. Our study shows that following mechanical injury human astrocytes produce IL-6, which may contribute to post-traumatic cerebrovascular dysfunction. Elucidating the precise role of intracerebral cytokines is essential to our understanding of the mechanism responsible for post-traumatic cerebrovascular dysfunction. |
Alternate Journal | Brain Res. |
PubMed ID | 8156401 |
Submitted by kej2006 on June 6, 2018 - 4:10pm