Title | Hand2 inhibits kidney specification while promoting vein formation within the posterior mesoderm. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Perens EA, Garavito-Aguilar ZV, Guio-Vega GP, Peña KT, Schindler YL, Yelon D |
Journal | Elife |
Volume | 5 |
Date Published | 2016 11 02 |
ISSN | 2050-084X |
Keywords | Animals, Basic Helix-Loop-Helix Transcription Factors, Body Patterning, Cell Differentiation, Gene Expression Regulation, Developmental, Kidney, Mesoderm, Mutation, Organogenesis, Transcription Factors, Veins, Zebrafish, Zebrafish Proteins |
Abstract | Proper organogenesis depends upon defining the precise dimensions of organ progenitor territories. Kidney progenitors originate within the intermediate mesoderm (IM), but the pathways that set the boundaries of the IM are poorly understood. Here, we show that the bHLH transcription factor Hand2 limits the size of the embryonic kidney by restricting IM dimensions. The IM is expanded in zebrafish mutants and is diminished when is overexpressed. Within the posterior mesoderm, is expressed laterally adjacent to the IM. Venous progenitors arise between these two territories, and promotes venous development while inhibiting IM formation at this interface. Furthermore, and the co-expressed zinc-finger transcription factor have functionally antagonistic influences on kidney development. Together, our data suggest that functions in opposition to to balance the formation of kidney and vein progenitors by regulating cell fate decisions at the lateral boundary of the IM. |
DOI | 10.7554/eLife.19941 |
Alternate Journal | Elife |
PubMed ID | 27805568 |
PubMed Central ID | PMC5132343 |
Grant List | R01 HL069594 / HL / NHLBI NIH HHS / United States R01 HL108599 / HL / NHLBI NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:11pm