Title | Widespread Inducible Transcription Downstream of Human Genes. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Vilborg A, Passarelli MC, Yario TA, Tycowski KT, Steitz JA |
Journal | Mol Cell |
Volume | 59 |
Issue | 3 |
Pagination | 449-61 |
Date Published | 2015 Aug 06 |
ISSN | 1097-4164 |
Keywords | Cell Line, Chromatin, Gene Expression Regulation, Genome, Human, Humans, Inositol 1,4,5-Trisphosphate Receptors, Osmotic Pressure, Poly A, Potassium Chloride, RNA, RNA, Long Noncoding, Sequence Analysis, RNA, Signal Transduction, Transcription, Genetic |
Abstract | Pervasive transcription of the human genome generates RNAs whose mode of formation and functions are largely uncharacterized. Here, we combine RNA-seq with detailed mechanistic studies to describe a transcript type derived from protein-coding genes. The resulting RNAs, which we call DoGs for downstream of gene containing transcripts, possess long non-coding regions (often >45 kb) and remain chromatin bound. DoGs are inducible by osmotic stress through an IP3 receptor signaling-dependent pathway, indicating active regulation. DoG levels are increased by decreased termination of the upstream transcript, a previously undescribed mechanism for rapid transcript induction. Relative depletion of polyA signals in DoG regions correlates with increased levels of DoGs after osmotic stress. We detect DoG transcription in several human cell lines and provide evidence for thousands of DoGs genome wide. |
DOI | 10.1016/j.molcel.2015.06.016 |
Alternate Journal | Mol. Cell |
PubMed ID | 26190259 |
PubMed Central ID | PMC4530028 |
Grant List | / / Howard Hughes Medical Institute / United States R01 GM026154 / GM / NIGMS NIH HHS / United States GM026154 / GM / NIGMS NIH HHS / United States |
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