Title | Live imaging of leukocyte recruitment in a zebrafish model of chemical liver injury. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Stoddard M, Huang C, Enyedi B, Niethammer P |
Journal | Sci Rep |
Volume | 9 |
Issue | 1 |
Pagination | 28 |
Date Published | 2019 01 10 |
ISSN | 2045-2322 |
Abstract | Studying early immune responses to organ damage in situ requires animal models amenable to intravital imaging. Here, we used transparent zebrafish larvae, a powerful animal model for innate immunity, to measure leukocyte recruitment to damaged livers. Bath application of metronidazole (Mtz) to fish expressing nitroreductase (NTR) under a liver-specific promoter damaged the organ within 24 hours causing oxidative stress, distorted liver morphology, accumulation of TUNEL-positive cells, and transcriptional upregulation of apoptotic and antioxidant genes. Inflammatory gene transcription in damaged hepatocytes was attenuated. In line with predominant apoptosis, macrophages were massively recruited into Mtz/NTR-damaged livers. By contrast, neutrophil infiltration was more variable and delayed, consistent with less abundant necrosis and an attenuated inflammatory capacity of damaged hepatocytes. |
DOI | 10.1038/s41598-018-36771-9 |
Alternate Journal | Sci Rep |
PubMed ID | 30631093 |
PubMed Central ID | PMC6328554 |
Grant List | P30 CA008748 / CA / NCI NIH HHS / United States R01 GM099970 / GM / NIGMS NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States |
Submitted by api_import on December 20, 2019 - 3:23pm