Title | Identification of receptors for pig endogenous retrovirus. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Ericsson TA, Takeuchi Y, Templin C, Quinn G, Farhadian SF, Wood JC, Oldmixon BA, Suling KM, Ishii JK, Kitagawa Y, Miyazawa T, Salomon DR, Weiss RA, Patience C |
Journal | Proc Natl Acad Sci U S A |
Volume | 100 |
Issue | 11 |
Pagination | 6759-64 |
Date Published | 2003 May 27 |
ISSN | 0027-8424 |
Keywords | Amino Acid Sequence, Animals, Base Sequence, DNA Primers, Endogenous Retroviruses, HeLa Cells, Humans, Mice, Molecular Sequence Data, Receptors, Virus, Sequence Homology, Amino Acid, Swine |
Abstract | Xenotransplantation of porcine tissues has the potential to treat a wide variety of major health problems including organ failure and diabetes. Balanced against the potential benefits of xenotransplantation, however, is the risk of human infection with a porcine microorganism. In particular, the transmission of porcine endogenous retrovirus (PERV) is a major concern [Chapman, L. E. & Bloom, E. T. (2001) J. Am. Med. Assoc. 285, 2304-2306]. Here we report the identification of two, sequence-related, human proteins that act as receptors for PERV-A, encoded by genes located on chromosomes 8 and 17. We also describe homologs from baboon and porcine cells that also are active as receptors. Conversely, activity could not be demonstrated with a syntenic murine receptor homolog. Sequence analysis indicates that PERV-A receptors [human PERV-A receptor (HuPAR)-1, HuPAR-2, baboon PERV-A receptor 2, and porcine PERV-A receptor] are multiple membrane-spanning proteins similar to receptors for other gammaretroviruses. Expression is widespread in human tissues including peripheral blood mononuclear cells, but their biological functions are unknown. The identification of the PERV-A receptors opens avenues of research necessary for a more complete assessment of the retroviral risks of pig to human xenotransplantation. |
DOI | 10.1073/pnas.1138025100 |
Alternate Journal | Proc. Natl. Acad. Sci. U.S.A. |
PubMed ID | 12740431 |
PubMed Central ID | PMC164520 |
Grant List | R01 AI045494 / AI / NIAID NIH HHS / United States R01 AI45494 / AI / NIAID NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:10pm