Title | IGDB-2, an Ig/FNIII protein, binds the ion channel LGC-34 and controls sensory compartment morphogenesis in C. elegans. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Wang W, Perens EA, Oikonomou G, Wallace SW, Lu Y, Shaham S |
Journal | Dev Biol |
Volume | 430 |
Issue | 1 |
Pagination | 105-112 |
Date Published | 2017 10 01 |
ISSN | 1095-564X |
Keywords | Alleles, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Compartmentation, Cell Membrane, Epistasis, Genetic, Genes, Suppressor, HEK293 Cells, Humans, Ligands, Models, Biological, Morphogenesis, Mutation, Neuroglia, Protein Binding, Protein Domains, Sensory Receptor Cells |
Abstract | Sensory organ glia surround neuronal receptive endings (NREs), forming a specialized compartment important for neuronal activity, and reminiscent of glia-ensheathed synapses in the central nervous system. We previously showed that DAF-6, a Patched-related protein, is required in glia of the C. elegans amphid sensory organ to restrict sensory compartment size. LIT-1, a Nemo-like kinase, and SNX-1, a retromer component, antagonize DAF-6 and promote compartment expansion. To further explore the machinery underlying compartment size control, we sought genes whose inactivation restores normal compartment size to daf-6 mutants. We found that mutations in igdb-2, encoding a single-pass transmembrane protein containing Ig-like and fibronectin type III domains, suppress daf-6 mutant defects. IGDB-2 acts in glia, where it localizes to glial membranes surrounding NREs, and, together with LIT-1 and SNX-1, regulates compartment morphogenesis. Immunoprecipitation followed by mass spectrometry demonstrates that IGDB-2 binds to LGC-34, a predicted ligand-gated ion channel, and lgc-34 mutations inhibit igdb-2 suppression of daf-6. Our findings reveal a novel membrane protein complex and suggest possible mechanisms for how sensory compartment size is controlled. |
DOI | 10.1016/j.ydbio.2017.08.009 |
Alternate Journal | Dev. Biol. |
PubMed ID | 28803967 |
PubMed Central ID | PMC5593787 |
Grant List | R01 NS064273 / NS / NINDS NIH HHS / United States P40 OD010440 / OD / NIH HHS / United States R01 NS081490 / NS / NINDS NIH HHS / United States R01 NS095795 / NS / NINDS NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States R01 HD078703 / HD / NICHD NIH HHS / United States |
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