Title | High-sensitivity flow cytometric analysis of mast cell clustering in systemic mastocytosis: a quantitative and statistical analysis. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Pozdnyakova O, LaPlante CD, Li B, Rosner B, Dorfman DM |
Journal | Leuk Lymphoma |
Volume | 56 |
Issue | 6 |
Pagination | 1735-41 |
Date Published | 2015 Jun |
ISSN | 1029-2403 |
Keywords | Bone Marrow Cells, Flow Cytometry, Humans, Immunophenotyping, Logistic Models, Mast Cells, Mastocytosis, Systemic, Proto-Oncogene Proteins c-kit, Reproducibility of Results, Sensitivity and Specificity |
Abstract | We characterized recently identified event clusters in systemic mastocytosis (SM) by calculating the coefficient of variation (CV, %) of CD117 and side scatter (SSC), based on the mean fluorescence intensity of mast cells using flow cytometry. Seventy-five samples were from patients with SM and 124 samples from patients negative for SM (non-SM). Discrete cluster formation seen in 50 cases correlated with significantly lower CV for SSC (46.1% vs. 61.0%, p < 0.0001) and CD117 (64.5% vs. 80.5%, p < 0.0001) for SM vs. non-SM samples. A combined CVCD117 + SSC of < 125 showed a sensitivity of 80% and specificity of 80% for SM with PPV of 67% and NPV of 80%. Probability scores of having SM, generated based on CVs for SSC and CD117, were significantly higher in patients with SM than non-SM (0.55 vs. 0.17, respectively; p < 0.001). Flow cytometric-based quantitative analysis of event clustering is a useful approach for diagnosing and monitoring patients with SM. |
DOI | 10.3109/10428194.2014.970546 |
Alternate Journal | Leuk. Lymphoma |
PubMed ID | 25284492 |
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