Title | Commensal microbes provide first line defense against infection. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Becattini S, Littmann ER, Carter RA, Kim SG, Morjaria SM, Ling L, Gyaltshen Y, Fontana E, Taur Y, Leiner IM, Pamer EG |
Journal | J Exp Med |
Volume | 214 |
Issue | 7 |
Pagination | 1973-1989 |
Date Published | 2017 Jul 03 |
ISSN | 1540-9538 |
Keywords | Animals, Anti-Bacterial Agents, Antibiosis, Feces, Gastrointestinal Microbiome, Host-Pathogen Interactions, Humans, Immunocompromised Host, Intestines, Listeria monocytogenes, Listeriosis, Mice, Inbred C57BL, Mice, Knockout, Survival Analysis, Survival Rate, Time Factors |
Abstract | is a foodborne pathogen that causes septicemia, meningitis and chorioamnionitis and is associated with high mortality. Immunocompetent humans and animals, however, can tolerate high doses of without developing systemic disease. The intestinal microbiota provides colonization resistance against many orally acquired pathogens, and antibiotic-mediated depletion of the microbiota reduces host resistance to infection. Here we show that a diverse microbiota markedly reduces colonization of the gut lumen and prevents systemic dissemination. Antibiotic administration to mice before low dose oral inoculation increases growth in the intestine. In immunodeficient or chemotherapy-treated mice, the intestinal microbiota provides nonredundant defense against lethal, disseminated infection. We have assembled a consortium of commensal bacteria belonging to the Clostridiales order, which exerts in vitro antilisterial activity and confers in vivo resistance upon transfer into germ free mice. Thus, we demonstrate a defensive role of the gut microbiota against infection and identify intestinal commensal species that, by enhancing resistance against this pathogen, represent potential probiotics. |
DOI | 10.1084/jem.20170495 |
Alternate Journal | J. Exp. Med. |
PubMed ID | 28588016 |
PubMed Central ID | PMC5502438 |
Grant List | K23 AI095398 / AI / NIAID NIH HHS / United States P30 CA008748 / CA / NCI NIH HHS / United States R01 AI042135 / AI / NIAID NIH HHS / United States U01 AI124275 / AI / NIAID NIH HHS / United States |
Submitted by api_import on June 6, 2018 - 4:10pm