Title | Cocaine exposure enhances permissiveness of quiescent T cells to HIV infection. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Kim SG, Jung JB, Dixit D, Rovner R, Zack JA, Baldwin GC, Vatakis DN |
Journal | J Leukoc Biol |
Volume | 94 |
Issue | 4 |
Pagination | 835-43 |
Date Published | 2013 Oct |
ISSN | 1938-3673 |
Keywords | Cocaine, HIV, HIV Infections, Humans, Kinetics, Phenotype, Receptors, Dopamine D4, Receptors, sigma, Reverse Transcription, RNA, Messenger, T-Lymphocytes, Viral Proteins, Virus Internalization |
Abstract | In vivo and in vitro exposure to stimulants has been associated with increased levels of HIV infection in PBMCs. Among these lymphocyte subsets, quiescent CD4(+) T cells make up the majority of circulating T cells in the blood. Others and we have demonstrated that HIV infects this population of cells inefficiently. However, minor changes in their cell state can render them permissive to infection, significantly impacting the viral reservoir. We have hypothesized that stimulants, such as cocaine, may perturb the activation state of quiescent cells enhancing permissiveness to infection. Quiescent T cells isolated from healthy human donors were exposed to cocaine and infected with HIV. Samples were harvested at different time-points to assess the impact of cocaine on their susceptibility to infection at various stages of the HIV life cycle. Our data show that a 3-day exposure to cocaine enhanced infection of quiescent cells, an effect that appears to be mediated by σ1R and D4R. Overall, our results indicate that cocaine-mediated effects on quiescent T cells may increase the pool of infection-susceptible T cells. The latter underscores the impact that stimulants have on HIV-seropositive individuals and the challenges posed for treatment. |
DOI | 10.1189/jlb.1112566 |
Alternate Journal | J. Leukoc. Biol. |
PubMed ID | 23817564 |
PubMed Central ID | PMC3774841 |
Grant List | R21DA031036-01A1 / DA / NIDA NIH HHS / United States R01 AI070010 / AI / NIAID NIH HHS / United States R01DA023386 / DA / NIDA NIH HHS / United States R01 DA023386 / DA / NIDA NIH HHS / United States R21 DA031036 / DA / NIDA NIH HHS / United States P30 AI028697 / AI / NIAID NIH HHS / United States R01AI070010 / AI / NIAID NIH HHS / United States 5P30 AI028697 / AI / NIAID NIH HHS / United States |
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