Title | Unique Organization of the Nuclear Envelope in the Post-natal Quiescent Neural Stem Cells. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Cebrián-Silla A, Alfaro-Cervelló C, Herranz-Pérez V, Kaneko N, Park DHwi, Sawamoto K, Alvarez-Buylla A, Lim DA, García-Verdugo JManuel |
Journal | Stem Cell Reports |
Volume | 9 |
Issue | 1 |
Pagination | 203-216 |
Date Published | 2017 07 11 |
ISSN | 2213-6711 |
Keywords | Adult Stem Cells, Animals, Astrocytes, Cell Cycle, Cells, Cultured, Chromatin, Lateral Ventricles, Mice, Neural Stem Cells, Nuclear Envelope |
Abstract | Neural stem cells (B1 astrocytes; NSCs) in the adult ventricular-subventricular-zone (V-SVZ) originate in the embryo. Surprisingly, recent work has shown that B1 cells remain largely quiescent. They are reactivated postnatally to function as primary progenitors for neurons destined for the olfactory bulb and some corpus callosum oligodendrocytes. The cellular and molecular properties of quiescent B1 cells remain unknown. Here we found that a subpopulation of B1 cells has a unique nuclear envelope invagination specialization similar to envelope-limited chromatin sheets (ELCS), reported in certain lymphocytes and some cancer cells. Using molecular markers, [H]thymidine birth-dating, and Ara-C, we found that B1 cells with ELCS correspond to quiescent NSCs. ELCS begin forming in embryonic radial glia cells and represent a specific nuclear compartment containing particular epigenetic modifications and telomeres. These results reveal a unique nuclear compartment in quiescent NSCs, which is useful for identifying these primary progenitors and study their gene regulation. |
DOI | 10.1016/j.stemcr.2017.05.024 |
Alternate Journal | Stem Cell Reports |
PubMed ID | 28648897 |
PubMed Central ID | PMC5511107 |
Submitted by kej2006 on June 6, 2018 - 4:12pm