Submitted by kej2006 on June 6, 2018 - 4:12pm
| Title | Tracking Plasma Cell Differentiation in Living Mice with Two-Photon Microscopy. |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | Ulbricht C, Lindquist RL, Tech L, Hauser AE |
| Journal | Methods Mol Biol |
| Volume | 1623 |
| Pagination | 37-50 |
| Date Published | 2017 |
| ISSN | 1940-6029 |
| Keywords | Animals, B-Lymphocytes, Cell Differentiation, Cell Tracking, Fluorescent Antibody Technique, Germinal Center, Image Processing, Computer-Assisted, Lymph Nodes, Mice, Mice, Transgenic, Microscopy, Plasma Cells, Time Factors |
| Abstract | Due to the multitude of cell types involved in the differentiation of plasma cells during the germinal center reaction, and due to a lack of in vitro systems, which recapitulate germinal centers, the most suitable way to study plasma cell generation in germinal centers is in vivo. In this chapter we describe how to induce humoral immune responses to defined model antigens and how to visualize and track plasma cells and their interactions with other cells in the lymph nodes of living mice. |
| DOI | 10.1007/978-1-4939-7095-7_3 |
| Alternate Journal | Methods Mol. Biol. |
| PubMed ID | 28589345 |