Title | Some, but not all, retromer components promote morphogenesis of C. elegans sensory compartments. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Oikonomou G, Perens EA, Lu Y, Shaham S |
Journal | Dev Biol |
Volume | 362 |
Issue | 1 |
Pagination | 42-9 |
Date Published | 2012 Feb 01 |
ISSN | 1095-564X |
Keywords | Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Membrane Proteins, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Morphogenesis, Multiprotein Complexes, Mutation, Neuroglia, Plasmids, Protein-Serine-Threonine Kinases, Sensory Receptor Cells, Sorting Nexins |
Abstract | The endings of sensory receptor cells often lie within specialized compartments formed by glial cells. The main sensory organ of Caenorhabditis elegans, the amphid, provides a powerful setting for studying glial compartment morphogenesis. Our previous studies showed that amphid compartment size is controlled by opposing activities of the Nemo-like kinase LIT-1, which promotes compartment expansion, and the Patched-related protein DAF-6, which restricts compartment growth. From a genetic screen for mutations able to suppress the bloated sensory compartments of daf-6 mutants, we identified an allele of the sorting nexin gene snx-1. SNX-1 protein is a component of the retromer, a protein complex that facilitates recycling of transmembrane proteins from the endosome to the Golgi network. We find that snx-1 functions cell autonomously within glia to promote sensory compartment growth, and that SNX-1 protein is enriched near the surface of the sensory compartment. snx-1 interacts genetically with lit-1 and another regulator of compartment size, the Dispatched-related gene che-14. Mutations in snx-3 and vps-29, also retromer genes, can suppress daf-6 defects. Surprisingly, however, remaining retromer components seem not to be involved. Our results suggest that a novel assembly of retromer components is important for determining sensory compartment dimensions. |
DOI | 10.1016/j.ydbio.2011.11.009 |
Alternate Journal | Dev. Biol. |
PubMed ID | 22138055 |
PubMed Central ID | PMC3254776 |
Grant List | R01 NS073121 / NS / NINDS NIH HHS / United States R01 NS064273 / NS / NINDS NIH HHS / United States R01 NS073121-03 / NS / NINDS NIH HHS / United States R01HD052677 / HD / NICHD NIH HHS / United States R01NS073121 / NS / NINDS NIH HHS / United States R01 HD052677-02 / HD / NICHD NIH HHS / United States R01 HD052677 / HD / NICHD NIH HHS / United States NS064273 / NS / NINDS NIH HHS / United States R01 NS064273-05 / NS / NINDS NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:11pm