A novel mouse strain optimized for chronic human antibody administration.

TitleA novel mouse strain optimized for chronic human antibody administration.
Publication TypeJournal Article
Year of Publication2022
AuthorsGupta A, Smith P, Bournazos S, Ravetch JV
JournalProc Natl Acad Sci U S A
Volume119
Issue10
Paginatione2123002119
Date Published2022 03 08
ISSN1091-6490
Abstract

Significance Species differences in IgG Fc-Fcγ receptor (FcγR) interactions have made humanized mouse models an attractive strategy to evaluate the efficacy and toxicity of human antibodies. We previously published a humanized FcγR mouse model that fully recapitulates the expression and function of these receptors in vivo. However, the immunogenicity of exogenous human IgG has made long-term assessment of antibody function challenging, since endogenous mouse anti-human IgG responses limit the duration and success of these studies. Here, we present a mouse strain that expresses human IgG1 and FcγRs, thereby conferring tolerance to chronic administration of human IgG and enabling functional assessment of antibodies. Because this strain is appropriate for chronic disease models, we expect that researchers will benefit from its use.

DOI10.1073/pnas.2123002119
Alternate JournalProc Natl Acad Sci U S A
PubMed ID35235456
PubMed Central IDPMC8915995
Grant ListR01 CA244327 / CA / NCI NIH HHS / United States
R35 CA196620 / CA / NCI NIH HHS / United States
T32 GM007739 / GM / NIGMS NIH HHS / United States
U19 AI111825 / AI / NIAID NIH HHS / United States

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