GuideScan software for improved single and paired CRISPR guide RNA design.

TitleGuideScan software for improved single and paired CRISPR guide RNA design.
Publication TypeJournal Article
Year of Publication2017
AuthorsPerez AR, Pritykin Y, Vidigal JA, Chhangawala S, Zamparo L, Leslie CS, Ventura A
JournalNat Biotechnol
Volume35
Issue4
Pagination347-349
Date Published2017 04
ISSN1546-1696
KeywordsAlgorithms, Chromosome Mapping, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems, Gene Silencing, Machine Learning, RNA, Small Interfering, Sequence Analysis, RNA, Software
Abstract

We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

DOI10.1038/nbt.3804
Alternate JournalNat. Biotechnol.
PubMed ID28263296
PubMed Central IDPMC5607865
Grant ListP30 CA008748 / CA / NCI NIH HHS / United States
U01 HG007033 / HG / NHGRI NIH HHS / United States
U01 HG007893 / HG / NHGRI NIH HHS / United States
U01 HG009395 / HG / NHGRI NIH HHS / United States

Person Type: