Submitted by kej2006 on June 6, 2018 - 4:10pm
Title | GuideScan software for improved single and paired CRISPR guide RNA design. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Perez AR, Pritykin Y, Vidigal JA, Chhangawala S, Zamparo L, Leslie CS, Ventura A |
Journal | Nat Biotechnol |
Volume | 35 |
Issue | 4 |
Pagination | 347-349 |
Date Published | 2017 04 |
ISSN | 1546-1696 |
Keywords | Algorithms, Chromosome Mapping, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems, Gene Silencing, Machine Learning, RNA, Small Interfering, Sequence Analysis, RNA, Software |
Abstract | We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools. |
DOI | 10.1038/nbt.3804 |
Alternate Journal | Nat. Biotechnol. |
PubMed ID | 28263296 |
PubMed Central ID | PMC5607865 |
Grant List | P30 CA008748 / CA / NCI NIH HHS / United States U01 HG007033 / HG / NHGRI NIH HHS / United States U01 HG007893 / HG / NHGRI NIH HHS / United States U01 HG009395 / HG / NHGRI NIH HHS / United States |