Title | Improved reference genome of Aedes aegypti informs arbovirus vector control. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Matthews BJ, Dudchenko O, Kingan SB, Koren S, Antoshechkin I, Crawford JE, Glassford WJ, Herre M, Redmond SN, Rose NH, Weedall GD, Wu Y, Batra SS, Brito-Sierra CA, Buckingham SD, Campbell CL, Chan S, Cox E, Evans BR, Fansiri T, Filipović I, Fontaine A, Gloria-Soria A, Hall R, Joardar VS, Jones AK, Kay RGG, Kodali VK, Lee J, Lycett GJ, Mitchell SN, Muehling J, Murphy MR, Omer AD, Partridge FA, Peluso P, Aiden APresser, Ramasamy V, Rašić G, Roy S, Saavedra-Rodriguez K, Sharan S, Sharma A, Smith MLaird, Turner J, Weakley AM, Zhao Z, Akbari OS, Black WC, Cao H, Darby AC, Hill CA, J Johnston S, Murphy TD, Raikhel AS, Sattelle DB, Sharakhov IV, White BJ, Zhao L, Aiden ELieberman, Mann RS, Lambrechts L, Powell JR, Sharakhova MV, Tu Z, Robertson HM, McBride CS, Hastie AR, Korlach J, Neafsey DE, Phillippy AM, Vosshall LB |
Journal | Nature |
Volume | 563 |
Issue | 7732 |
Pagination | 501-507 |
Date Published | 2018 11 |
ISSN | 1476-4687 |
Keywords | Aedes, Animals, Arbovirus Infections, Arboviruses, Dengue Virus, DNA Copy Number Variations, Female, Genetic Variation, Genetics, Population, Genome, Insect, Genomics, Glutathione Transferase, Insect Control, Insecticide Resistance, Male, Molecular Sequence Annotation, Mosquito Vectors, Multigene Family, Pyrethrins, Reference Standards, Sex Determination Processes |
Abstract | Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector. |
DOI | 10.1038/s41586-018-0692-z |
Alternate Journal | Nature |
PubMed ID | 30429615 |
PubMed Central ID | PMC6421076 |
Grant List | U01 HL130010 / HL / NHLBI NIH HHS / United States UL1 TR000043 / TR / NCATS NIH HHS / United States F30 DC017658 / DC / NIDCD NIH HHS / United States R01 AI121211 / AI / NIAID NIH HHS / United States R35 GM118336 / GM / NIGMS NIH HHS / United States K22 AI113060 / AI / NIAID NIH HHS / United States UM1 HG009375 / HG / NHGRI NIH HHS / United States R01 DC014247 / DC / NIDCD NIH HHS / United States R01 AI101112 / AI / NIAID NIH HHS / United States R01 AI123338 / AI / NIAID NIH HHS / United States DP2 OD008540 / OD / NIH HHS / United States MC_PC_16078 / MRC_ / Medical Research Council / United Kingdom T32 GM007739 / GM / NIGMS NIH HHS / United States R21 AI121853 / AI / NIAID NIH HHS / United States R21 AI123937 / AI / NIAID NIH HHS / United States U19 AI110818 / AI / NIAID NIH HHS / United States BB/F021933/1 / BB_ / Biotechnology and Biological Sciences Research Council / United Kingdom |
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