Title | HIV Detection via a Carbon Nanotube RNA Sensor. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Harvey JD, Baker HA, Ortiz MV, Kentsis A, Heller DA |
Journal | ACS Sens |
Volume | 4 |
Issue | 5 |
Pagination | 1236-1244 |
Date Published | 2019 05 24 |
ISSN | 2379-3694 |
Abstract | Viral illnesses remain a significant concern in global health. Rapid and quantitative early detection of viral oligonucleotides without the need for purification, amplification, or labeling would be valuable in guiding successful treatment strategies. Single-walled carbon nanotube-based sensors recently demonstrated optical detection of small, free oligonucleotides in biofluids and in vivo, although proteins diminished sensitivity. Here, we discovered an unexpected phenomenon wherein the carbon nanotube optical response to nucleic acids can be enhanced by denatured proteins. Mechanistic studies found that hydrophobic patches of the denatured protein chain interact with the freed nanotube surface after hybridization, resulting in enhanced shifting of the nanotube emission. We employed this mechanism to detect an intact HIV in serum, resulting in specific responses within minutes. This work portends a route toward point-of-care optical detection of viruses or other nucleic acid-based analytes. |
DOI | 10.1021/acssensors.9b00025 |
Alternate Journal | ACS Sens |
PubMed ID | 31056899 |
Grant List | DP2 HD075698 / HD / NICHD NIH HHS / United States P30 CA008748 / CA / NCI NIH HHS / United States K12 CA184746 / CA / NCI NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States |
Submitted by api_import on December 2, 2019 - 10:24am