Title | Translocation capture sequencing: a method for high throughput mapping of chromosomal rearrangements. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Oliveira TY, Resch W, Jankovic M, Casellas R, Nussenzweig MC, Klein IA |
Journal | J Immunol Methods |
Volume | 375 |
Issue | 1-2 |
Pagination | 176-81 |
Date Published | 2012 Jan 31 |
ISSN | 1872-7905 |
Keywords | Animals, B-Lymphocytes, Chromosome Mapping, Cytidine Deaminase, DNA Breaks, Double-Stranded, DNA Damage, Genes, myc, Genome-Wide Association Study, Immunoglobulin Heavy Chains, Lymphoma, B-Cell, Mice, Sequence Analysis, DNA, Translocation, Genetic |
Abstract | Chromosomal translocations require formation and joining of DNA double strand breaks (DSBs). These events disrupt the integrity of the genome and are involved in producing leukemias, lymphomas and sarcomas. Translocations are frequent, clonal and recurrent in mature B cell lymphomas, which bear a particularly high DNA damage burden by virtue of activation-induced cytidine deaminase (AID) expression. Despite the ubiquity of genomic rearrangements, the forces that underlie their genesis are not well understood. Here, we provide a detailed description of a new method for studying these events, translocation capture sequencing (TC-Seq). TC-Seq provides the means to document chromosomal rearrangements genome-wide in primary cells, and to discover recombination hotspots. Demonstrating its effectiveness, we successfully estimate the frequency of c-myc/IgH translocations in primary B cells, and identify hotspots of AID-mediated recombination. Furthermore, TC-Seq can be adapted to generate genome-wide rearrangement maps in any cell type and under any condition. |
DOI | 10.1016/j.jim.2011.10.007 |
Alternate Journal | J. Immunol. Methods |
PubMed ID | 22033343 |
PubMed Central ID | PMC3285106 |
Grant List | R01 AI037526 / AI / NIAID NIH HHS / United States GM07739 / GM / NIGMS NIH HHS / United States / / Intramural NIH HHS / United States R01 AI037526-17 / AI / NIAID NIH HHS / United States / / Howard Hughes Medical Institute / United States AI037526 / AI / NIAID NIH HHS / United States R37 AI037526 / AI / NIAID NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:11pm