Title | Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Nowakowski TJ, Bhaduri A, Pollen AA, Alvarado B, Mostajo-Radji MA, Di Lullo E, Haeussler M, Sandoval-Espinosa C, Liu SJohn, Velmeshev D, Ounadjela JRyad, Shuga J, Wang X, Lim DA, West JA, Leyrat AA, W Kent J, Kriegstein AR |
Journal | Science |
Volume | 358 |
Issue | 6368 |
Pagination | 1318-1323 |
Date Published | 2017 12 08 |
ISSN | 1095-9203 |
Keywords | Cerebral Cortex, Gene Expression Regulation, Developmental, Humans, Neurogenesis, Neuroglia, Neurons, Telencephalon |
Abstract | Systematic analyses of spatiotemporal gene expression trajectories during organogenesis have been challenging because diverse cell types at different stages of maturation and differentiation coexist in the emerging tissues. We identified discrete cell types as well as temporally and spatially restricted trajectories of radial glia maturation and neurogenesis in developing human telencephalon. These lineage-specific trajectories reveal the expression of neurogenic transcription factors in early radial glia and enriched activation of mammalian target of rapamycin signaling in outer radial glia. Across cortical areas, modest transcriptional differences among radial glia cascade into robust typological distinctions among maturing neurons. Together, our results support a mixed model of topographical, typological, and temporal hierarchies governing cell-type diversity in the developing human telencephalon, including distinct excitatory lineages emerging in rostral and caudal cerebral cortex. |
DOI | 10.1126/science.aap8809 |
Alternate Journal | Science |
PubMed ID | 29217575 |
Grant List | U01 MH114825 / MH / NIMH NIH HHS / United States U01 MH105989 / MH / NIMH NIH HHS / United States R35 NS097305 / NS / NINDS NIH HHS / United States R01 NS091544 / NS / NINDS NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:13pm