| Title | NITRIC OXIDE INHIBITORY ACTIVITY OF (LOGANIACEAE) LEAF EXTRACTS AND FRACTIONS. |
| Publication Type | Journal Article |
| Year of Publication | 2016 |
| Authors | Ai I, Jp D, Sa A, Mm S, Jn E |
| Journal | Afr J Tradit Complement Altern Med |
| Volume | 13 |
| Issue | 6 |
| Pagination | 22-26 |
| Date Published | 2016 |
| ISSN | 2505-0044 |
| Keywords | Anti-Inflammatory Agents, Chemical Fractionation, Humans, Nitric Oxide, Plant Extracts, Plant Leaves, Strychnos, U937 Cells |
| Abstract | BACKGROUND: The study was aimed at determining the anti-inflammatory activity of fractions and extracts obtained from leaves on a mediator of inflammation nitric oxide (NO). MATERIALS AND METHODS: Leaves were extracted with acetone and separated into fractions with different polarities by solvent-solvent fractionation. The Griess assay was used to determine the nitric oxide (NO) inhibitory activity. Cellular toxicity was determined by "using the MTT reduction assay". RESULTS: With the exception of the ethyl acetate fraction which had an IC >750 μg/mL, all extracts and fractions had significant nitric oxide-inhibitory activity. The most active being the water fraction, chloroform fraction and the dichloromethane/methanol extracts with IC values of 88.43 μg/mL, 96.72 μg/mL and 115.62 μg/mL, respectively. The extracts and fractions had low cytotoxicity on macrophage U937 cell lines. CONCLUSION: Extracts and fractions of leaves may be promising sources of natural anti-inflammatory agents. Findings obtained from this study showed that leaves possess promising anti-inflammatory action and could be used in the treatment of inflammation-related conditions. |
| DOI | 10.21010/ajtcam.v13i6.5 |
| Alternate Journal | Afr J Tradit Complement Altern Med |
| PubMed ID | 28480356 |
| PubMed Central ID | PMC5412197 |
Submitted by kej2006 on June 6, 2018 - 4:12pm