Title | Murine Kupffer cells. Mononuclear phagocytes deficient in the generation of reactive oxygen intermediates. |
Publication Type | Journal Article |
Year of Publication | 1985 |
Authors | Lepay DA, Nathan CF, Steinman RM, Murray HW, Cohn ZA |
Journal | J Exp Med |
Volume | 161 |
Issue | 5 |
Pagination | 1079-96 |
Date Published | 1985 May 01 |
ISSN | 0022-1007 |
Keywords | Animals, Carbon, Cell Separation, Female, Free Radicals, Histocompatibility Antigens Class II, Interferon-gamma, Kupffer Cells, Leishmania, Lymphokines, Mice, Mice, Inbred BALB C, Oxygen, Oxygen Consumption, Peptide Biosynthesis, Phagocytosis, Phenotype, Toxoplasma |
Abstract | Murine Kupffer cells (KC) were isolated by a high yield collagenase perfusion technique. The morphology, surface markers, and secretory products were typical of macrophages in other tissues. However, KC released negligible levels of H2O2 and O-2, in contrast to peritoneal macrophages. KC oxygen consumption was not increased by agents triggering a respiratory burst in peritoneal cells. Moreover, KC capacity to secrete reactive oxygen intermediates (ROI), in contrast to Ia antigen expression, was not enhanced by exposure to lymphokines or recombinant gamma interferon. The selective defect in KC oxidative response was paralleled by impaired in vitro killing of Toxoplasma gondii trophozoites and Leishmania donovani promastigotes and amastigotes. Deficient secretion of ROI by KC might protect hepatocytes and erythrocytes from injury during endocytosis by KC, but might render the liver more susceptible to parasitization by organisms that are primarily killed through oxygen-dependent mechanisms. |
Alternate Journal | J. Exp. Med. |
PubMed ID | 3921651 |
PubMed Central ID | PMC2187591 |
Grant List | AI 07012 / AI / NIAID NIH HHS / United States AI 13013 / AI / NIAID NIH HHS / United States AI 16963 / AI / NIAID NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:10pm