Title | The microanatomic segregation of selection by apoptosis in the germinal center. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Mayer CT, Gazumyan A, Kara EE, Gitlin AD, Golijanin J, Viant C, Pai J, Oliveira TY, Wang Q, Escolano A, Medina-RamÃrez M, Sanders RW, Nussenzweig MC |
Journal | Science |
Volume | 358 |
Issue | 6360 |
Date Published | 2017 10 13 |
ISSN | 1095-9203 |
Keywords | Animals, Antibodies, Monoclonal, Apoptosis, B-Lymphocytes, Cell Division, Cytidine Deaminase, Germinal Center, Immunoglobulin Class Switching, Mice, Mice, Inbred C57BL, Mice, Transgenic, Receptors, Antigen, B-Cell |
Abstract | B cells undergo rapid cell division and affinity maturation in anatomically distinct sites in lymphoid organs called germinal centers (GCs). Homeostasis is maintained in part by B cell apoptosis. However, the precise contribution of apoptosis to GC biology and selection is not well defined. We developed apoptosis-indicator mice and used them to visualize, purify, and characterize dying GC B cells. Apoptosis is prevalent in the GC, with up to half of all GC B cells dying every 6 hours. Moreover, programmed cell death is differentially regulated in the light zone and the dark zone: Light-zone B cells die by default if they are not positively selected, whereas dark-zone cells die when their antigen receptors are damaged by activation-induced cytidine deaminase. |
DOI | 10.1126/science.aao2602 |
Alternate Journal | Science |
PubMed ID | 28935768 |
PubMed Central ID | PMC5957278 |
Grant List | R01 AI037526 / AI / NIAID NIH HHS / United States UM1 AI100663 / AI / NIAID NIH HHS / United States P01 AI100148 / AI / NIAID NIH HHS / United States / / Howard Hughes Medical Institute / United States T32 GM007739 / GM / NIGMS NIH HHS / United States F30 AI109903 / AI / NIAID NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:12pm