Title | Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Howlett RM, Davey MP, Kelly DJ |
Journal | Methods Mol Biol |
Volume | 1512 |
Pagination | 189-197 |
Date Published | 2017 |
ISSN | 1940-6029 |
Keywords | Campylobacter jejuni, Flow Injection Analysis, Metabolome, Metabolomics, Principal Component Analysis, Spectrometry, Mass, Electrospray Ionization |
Abstract | Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the high-throughput and relatively simple sample preparation of DIMS has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. Here we describe a metabolomic fingerprinting approach with sampling and extraction methodologies optimized for direct-injection electrospray ionization mass spectrometry (ESI-MS), which we have used as a means of comparing wild-type and isogenic mutant strains of C. jejuni with various metabolic blocks. |
DOI | 10.1007/978-1-4939-6536-6_16 |
Alternate Journal | Methods Mol. Biol. |
PubMed ID | 27885608 |
Grant List | / / Biotechnology and Biological Sciences Research Council / United Kingdom 083772/Z/07/Z / / Wellcome Trust / United Kingdom |
Submitted by kej2006 on June 6, 2018 - 4:11pm