Submitted by kej2006 on June 6, 2018 - 4:12pm
Title | Determining Diguanylate Cyclase Activity (Radioactive Assay). |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Kazmierczak BI |
Journal | Methods Mol Biol |
Volume | 1657 |
Pagination | 285-289 |
Date Published | 2017 |
ISSN | 1940-6029 |
Keywords | Chromatography, Thin Layer, Enzyme Activation, Enzyme Assays, Escherichia coli Proteins, Phosphorus Radioisotopes, Phosphorus-Oxygen Lyases |
Abstract | Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography. |
DOI | 10.1007/978-1-4939-7240-1_22 |
Alternate Journal | Methods Mol. Biol. |
PubMed ID | 28889302 |