Title | A common assembly module in injectisome and flagellar type III secretion sorting platforms. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Notti RQ, Bhattacharya S, Lilic M, C Stebbins E |
Journal | Nat Commun |
Volume | 6 |
Pagination | 7125 |
Date Published | 2015 May 21 |
ISSN | 2041-1723 |
Keywords | Amino Acid Sequence, Flagella, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Protein Structure, Tertiary, Salmonella typhimurium, Type III Secretion Systems |
Abstract | Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design. |
DOI | 10.1038/ncomms8125 |
Alternate Journal | Nat Commun |
PubMed ID | 25994170 |
PubMed Central ID | PMC4443714 |
Grant List | CO6RR015495 / CO / NCI NIH HHS / United States T32GM07739 / GM / NIGMS NIH HHS / United States P41 GM103473 / GM / NIGMS NIH HHS / United States P41GM066354 / GM / NIGMS NIH HHS / United States R01 EB000998 / EB / NIBIB NIH HHS / United States S10 RR022321 / RR / NCRR NIH HHS / United States C06 RR015495 / RR / NCRR NIH HHS / United States P41 GM111244 / GM / NIGMS NIH HHS / United States AI093704 / AI / NIAID NIH HHS / United States 1S10RR022321-01 / RR / NCRR NIH HHS / United States P30-EB-00998 / EB / NIBIB NIH HHS / United States P41 GM066354 / GM / NIGMS NIH HHS / United States T32 GM007739 / GM / NIGMS NIH HHS / United States R56 AI052182 / AI / NIAID NIH HHS / United States AI52182 / AI / NIAID NIH HHS / United States R01 AI052182 / AI / NIAID NIH HHS / United States P41GM103473 / GM / NIGMS NIH HHS / United States R56 AI093704 / AI / NIAID NIH HHS / United States |
Submitted by kej2006 on June 6, 2018 - 4:11pm