IGDB-2, an Ig/FNIII protein, binds the ion channel LGC-34 and controls sensory compartment morphogenesis in C. elegans.

TitleIGDB-2, an Ig/FNIII protein, binds the ion channel LGC-34 and controls sensory compartment morphogenesis in C. elegans.
Publication TypeJournal Article
Year of Publication2017
AuthorsWang W, Perens EA, Oikonomou G, Wallace SW, Lu Y, Shaham S
JournalDev Biol
Volume430
Issue1
Pagination105-112
Date Published2017 10 01
ISSN1095-564X
KeywordsAlleles, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cell Compartmentation, Cell Membrane, Epistasis, Genetic, Genes, Suppressor, HEK293 Cells, Humans, Ligands, Models, Biological, Morphogenesis, Mutation, Neuroglia, Protein Binding, Protein Domains, Sensory Receptor Cells
Abstract

Sensory organ glia surround neuronal receptive endings (NREs), forming a specialized compartment important for neuronal activity, and reminiscent of glia-ensheathed synapses in the central nervous system. We previously showed that DAF-6, a Patched-related protein, is required in glia of the C. elegans amphid sensory organ to restrict sensory compartment size. LIT-1, a Nemo-like kinase, and SNX-1, a retromer component, antagonize DAF-6 and promote compartment expansion. To further explore the machinery underlying compartment size control, we sought genes whose inactivation restores normal compartment size to daf-6 mutants. We found that mutations in igdb-2, encoding a single-pass transmembrane protein containing Ig-like and fibronectin type III domains, suppress daf-6 mutant defects. IGDB-2 acts in glia, where it localizes to glial membranes surrounding NREs, and, together with LIT-1 and SNX-1, regulates compartment morphogenesis. Immunoprecipitation followed by mass spectrometry demonstrates that IGDB-2 binds to LGC-34, a predicted ligand-gated ion channel, and lgc-34 mutations inhibit igdb-2 suppression of daf-6. Our findings reveal a novel membrane protein complex and suggest possible mechanisms for how sensory compartment size is controlled.

DOI10.1016/j.ydbio.2017.08.009
Alternate JournalDev. Biol.
PubMed ID28803967
PubMed Central IDPMC5593787
Grant ListR01 NS064273 / NS / NINDS NIH HHS / United States
P40 OD010440 / OD / NIH HHS / United States
R01 NS081490 / NS / NINDS NIH HHS / United States
R01 NS095795 / NS / NINDS NIH HHS / United States
T32 GM007739 / GM / NIGMS NIH HHS / United States
R01 HD078703 / HD / NICHD NIH HHS / United States

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